|
KMID : 0377619880530100693
|
|
Korean Jungang Medical Journal
1988 Volume.53 No. 10 p.693 ~ p.699
|
|
|
Restriction and Modification System in Brevibacterium divaricatum I
|
|
|
|
|
Abstract
|
|
|
The isolation and, characterization of a new type II restriction endonuclease (Bdi I) from Brevibacterium divaricatum KCT 8035 were described. Bdi I. endonuclease was isolated by the following procedures; ammonium sulfate fractionation, DEAE-cellulose chromatography, phosphocellulose chromatography and hydroxylapatite chromatography.
The isolated Bdi I restriction endonuclease was an isoschizomer of Cla I which recognizes 5 ATCGAT 3 , and cleaves between T and C within the recognition seguence. The optimal temperature on the enzyme activity was 37°C and optimal salt (NaCl) concentration was 50-100mM. Also Bdi I endonuclease reguired Mgr as a cofactor.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|